Summary
Type: SPE
Application #: 301110
Catalog#: 730011 - SPE Cartridges, Chromabond, C18 ec, 1ml, PP, 100mg, 100/pk
Detection:
Conditions: Column conditioning:
1 ml methanol, then 1 ml dist. water
Sample application:
Slowly pour 400 μl pretreated sample through the column
Column washing:
1 ml H2O, 1 ml CH3CN / H2O (1 : 1, v/v)
Elution:
acetonitrile / methanol / buffer A (1 : 2 : 1, v/v/v), pH 4.1 [buffer A: 4.5 ml
85 % phosphoric acid and 4.5 ml triethylamine filled up to 1000 ml with water
(pH 2.2)]
Authors: Macherey-Nagel
Source: LC Department